roxS [2017-04-28 10:33:02]

small RNA, controls expression of [gene|B729EFC0BF0D75765C2D2B0B4F397E0AEE11F1E7|ytdI], [gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]-[gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD], and [gene|search|yflS ]

function: control of [gene|B729EFC0BF0D75765C2D2B0B4F397E0AEE11F1E7|ytdI], [gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]-[gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD], and [gene|search|yflS ]expression

product: trans-acting regulatory RNA

essential: no

synonyms: rsaE

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.9|NcRNA] → [category|SW 6.9.5|Regulatory RNAs]

Expression and Regulation

Operons

• genes: [gene|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS]

  description

  • [pubmed|22383849]

  regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: transcriptional activation, [pubmed|22383849], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]
  • [protein|B5EF521437323EF43F08E5EFDB5C798616CA499A|Rex]: repression, [pubmed|28436820], in [regulon|B5EF521437323EF43F08E5EFDB5C798616CA499A|Rex regulon]

  regulation

  • induced by nitric oxide (NO) ([protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]) [pubmed|22383849]
  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: transcriptional activation [pubmed|22383849]
  • [protein|B5EF521437323EF43F08E5EFDB5C798616CA499A|Rex]: transcriptional repression [pubmed|28436820]

  additional information

  • the [gene|search|roxS ]RNA is degraded by the joined activities of RNase Y (initial endonucleolytic cleavage) and RNase J1 (processive degradation) [pubmed|22383849]
  • the [gene|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS]-[gene|search|ppnKB ]and [gene|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS]-[gene|search|sucC ]RNA duplexes form a cleavage site for RNase III [pubmed|22383849]

Biological materials

mutant

• available in [SW|Ciaran Condon]'s lab [pubmed|25643072]

References

Research papers

• 25643072,28436820

The RNA

Catalyzed reaction/ biological activity

• hybridizes to [gene|B729EFC0BF0D75765C2D2B0B4F397E0AEE11F1E7|ytdI] and [gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD] mRNAs to inhibit their translation and to destabilize them [pubmed|25643072]
• binds the 5' end of the [gene|73356B73D6C89D6569CECCC7295AF85CC7A1E6E3|yflS] mRNA to prevent its degradation by [SW|rnjA|RNase J1] [pubmed|28436820]